What is 10x PBS Buffer used for?

10x PBS Buffer is primarily used in biological and biochemical research as a buffering agent. It helps maintain a stable pH level when diluting samples, making it ideal for cell culture, the dilution of proteins, and washing of cells. Its composition of sodium phosphate, sodium chloride, and potassium chloride provides ionic strength, which is crucial for assays, such as Western blots and ELISA. For example, when preparing cell cultures, using PBS ensures that the cells remain healthy and viable for experimentation.

How do you prepare a working solution from 10x PBS Buffer?

To prepare a working solution from 10x PBS Buffer, simply dilute it with distilled water. For most applications, a common dilution used is 1 part 10x PBS to 9 parts water, resulting in a 1x PBS solution. Make sure to mix thoroughly. This dilution is ready for use in applications such as washing cells before experiments or as a diluent for biological samples. This preparation process is straightforward, ensuring that you can have a consistent buffer for various experimental procedures.

Is 10x PBS Buffer suitable for mammalian cell culture?

Yes, 10x PBS Buffer is highly suitable for mammalian cell culture. It provides the necessary ionic environment to stabilize the cells during various biological processes. In cell culture, PBS is often used for rinsing the cells, removing media, or resuspending them without causing damage. As a result, it ensures that cells remain in optimal condition for experiments and assays, enhancing the reliability of your results.

What is the difference between 1x PBS and 10x PBS?

The main difference between 1x PBS and 10x PBS lies in their concentration. 10x PBS is ten times more concentrated than the 1x formulation. The 10x PBS buffer is typically diluted before use to achieve the desired concentration of 1x, which is the standard working concentration for many laboratory applications. For instance, if you're conducting an experiment that requires a washing step, you’ll use the diluted 1x PBS to ensure optimal pH and ionic strength without affecting your results.

Can 10x PBS Buffer be used for protein storage?

While 10x PBS Buffer is excellent for preparing solutions, it is not ideal for long-term protein storage. Proteins are generally more stable in solutions that contain stabilizers such as glycerol or specific buffer conditions tailored for the protein's stability. Instead, 10x PBS can be used to dilute proteins for immediate use in assays or experiments. Always consider the nature of your protein and consult relevant literature to choose the best storage conditions.

How should 10x PBS Buffer be stored?

10x PBS Buffer should be stored at room temperature or in a refrigerator, depending on the specific formulation and any added components. Avoid exposure to extreme conditions that might alter its pH or ionic concentrations. Once opened, it’s essential to keep it tightly sealed to prevent contamination. Proper storage ensures that the buffer remains effective for various laboratory applications, thus maintaining the integrity of your experimental results.

Is 10x PBS Buffer toxic?

10x PBS Buffer is generally considered non-toxic and safe for handling in laboratory environments. However, standard laboratory safety protocols should always be followed. Use gloves and goggles when handling any chemical solutions to protect yourself from potential irritants. Despite its safety profile, awareness of proper disposal methods and material safety data sheets (MSDS) for the components used in PBS can ensure safe handling practices.

What are the typical applications of 10x PBS Buffer in research?

10x PBS Buffer is utilized in a wide range of research applications, including cell washing, sample dilution, and protein assays. It is commonly used in immunological techniques, such as ELISA and Western blots, where maintaining a stable pH and ionic strength is crucial. Additionally, it's indispensable in the preparation and dilution of biological samples for microscopy and flow cytometry. This versatility makes 10x PBS a fundamental tool in many research laboratories.

Can I use 10x PBS Buffer with live cell imaging?

Yes, 10x PBS Buffer can be used for live cell imaging; however, it must first be diluted to a 1x concentration to avoid hyperosmolarity that could affect cell health. In live cell imaging experiments, 1x PBS can serve as a wash buffer before adding imaging media. Its balanced ionic strength helps maintain cell integrity during microscopy, making it suitable for various imaging techniques, including fluorescence and confocal microscopy.

Where can I buy 10x PBS Buffer (1 L) in Estonia?

You can buy 10x PBS Buffer (1 L) from Ubuy in Estonia. Ubuy offers a wide selection of laboratory reagents and supplies, making it easy to find quality products like 10x PBS. So, if you're looking to purchase this essential buffer, Ubuy is your go-to source for reliable lab supplies.

Kuidas osta 10x PBS Buffer (1 L) Internetist Ubuyst?

Ubuyst on lihtne veebis osta 10x PBS Buffer (1 L).. Peate lihtsalt otsima toote, valima väljaregistreerimise ajal tarneviisi ja hankima selle teie asukohta.

Kas 10x PBS Buffer (1 L) on võrgus ostmiseks saadaval asukohas Estonia?

Jah, Ubuy Estonia-s on see toode teile mõistliku hinnaga ostmiseks saadaval.. 10x PBS Buffer (1 L) pole kohapeal saadaval, kuid võite usaldada meile meie kiirsaadetmise teenused.

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Each 10X PBS solution is ready to use upon dilution to the desired concentration.

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10x PBS Buffer (1 L)

Toote nr: 90238222

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What Stands Out

Concentration

The 10x PBS Buffer offers a high concentration solution, ensuring reliable dilutions for various biological applications, thereby saving time and reducing the need for frequent preparation.

Versatility

Ideal for cell culture, immunoassays, and molecular biology, this buffer is versatile enough to meet diverse laboratory needs, enhancing overall experimental efficiency.

Convenient Packaging

Packaged in a 1-liter bottle for ease of use, this 10x PBS Buffer reduces handling time and minimizes contamination risk, ensuring a consistent supply for all your research activities.

Toote üksikasjad

Brand Name	Generic
Manufacturer	Bioland Scientific LLC
Model Number	PBS01-1L
Part Number	PBS01-1L
Included Components	buffer
Item Weight	2.2 lbs (1 kg)

Who Should Buy?

Molecular Biologists
Ideal for molecular biology applications such as dilution, washing, and buffer for various experiments.
Cell Culture Users
Supports cell culture by maintaining osmotic balance and providing essential ions for cell health.
Immunologists
Useful in immunological assays like ELISA and for diluting antibodies, ensuring reliable results.

Field Researchers
Not ideal for field applications where stable conditions and lab-grade reagents are unavailable.

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10x PBS Buffer (1 L)

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küsimus: Kuidas osta 10x PBS Buffer (1 L) Internetist Ubuyst?

vastama: Ubuyst on lihtne veebis osta 10x PBS Buffer (1 L).. Peate lihtsalt otsima toote, valima väljaregistreerimise ajal tarneviisi ja hankima selle teie asukohta.
küsimus: Kas 10x PBS Buffer (1 L) on võrgus ostmiseks saadaval asukohas Estonia?

vastama: Jah, Ubuy Estonia-s on see toode teile mõistliku hinnaga ostmiseks saadaval.. 10x PBS Buffer (1 L) pole kohapeal saadaval, kuid võite usaldada meile meie kiirsaadetmise teenused.
küsimus: Kui kaua kulub toote kättesaamiseks pärast tellimuse esitamist?

vastama: Teie tellitud toote tarneaeg sõltub teie tellitud kaubast ja teie valitud saatmisviisist.. Hinnanguline tarneaeg mainitakse kassaprotsessi ajal, seega olge ostlemisel muretu.

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Ready-to-use 10X PBS solution for your laboratory needs.
Convenient dilution to 1X working concentration for quick application.
Ideal for Western blot, ELISA, and immunoprecipitation.
Formulated with ultrapure-grade phosphate buffers and saline solutions.
Clear colorless liquid with a pH of 7.4 ± 0.2.
Filtered with a 0.45µm filter ensuring high purity.

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10x PBS Buffer (1 L)

€ 49

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Toote üksikasjad

Who Should Buy?

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Klientide küsimused ja vastused

Buffers PBS01-1L Editorial Review

Customer Reviews & Ratings

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€ 49

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